New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids.
Yeast
; 20(11): 985-93, 2003 Aug.
Article
in En
| MEDLINE
| ID: mdl-12898713
ABSTRACT
Marker swap plasmids can be used to change markers for genes disrupted with nutritional markers in the yeast Saccharomyces cerevisiae. We describe 18 new marker swap plasmids, and we also review other plasmids available for marker conversions. All of these plasmids have long regions of flanking sequence identity, and thus the efficiency of homologous recombination mediated by marker conversion is very high. Marker swaps allow one to easily perform crosses to construct double mutant strains even if each of the disrupted strains contains the same marker, as is the case with the KanMX marker used in the yeast knockout collection. Marker swaps can also be used to change the selectable marker on plasmids, eliminating the need for subcloning.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Saccharomyces cerevisiae
/
Genetic Markers
/
Mutagenesis, Insertional
Language:
En
Journal:
Yeast
Journal subject:
MICROBIOLOGIA
Year:
2003
Document type:
Article
Affiliation country: